Mitosis Execution
Gene co-expression module in Gamma-delta T cells
| Category | Cell cycle |
|---|---|
| Genes | 21 |
| Annotation certainty | 5 of 5 |
| Annotation consistency | 20 of 21 genes have a known function matching the annotation |
Why this annotation
All hub genes are canonical late G2/M markers: MKI67 (Ki-67, mitosis marker), TPX2 (spindle assembly factor), NUSAP1 (spindle-associated), TOP2A (topoisomerase II alpha, chromosome condensation/segregation), CENPF (centromere protein F/kinetochore), CENPE (kinetochore motor), NDC80 (kinetochore-microtubule attachment), BUB1 (spindle assembly checkpoint kinase), MAD2L1 (SAC), PRC1 (central spindle/cytokinesis), RACGAP1 (cytokinesis), CDCA8 (chromosomal passenger complex/aurora B), RRM2 (ribonucleotide reductase M2, S/G2), CKS1B (CDK regulatory, G2/M), ASF1B (histone chaperone, G2/M), UBE2T (Fanconi pathway ubiquitin E2, G2), EZH2 (PRC2, proliferating cells), NCAPG2/NCAPH (condensin II). This is the most mitotically committed module in the batch. Extremely low mean expression (0.022) and 1.9% positive cells confirm rare actively-dividing cells.
Genes
ASF1B, BUB1, CDCA8, CENPE, CENPF, CKS1B, EZH2, MAD2L1, MKI67, NCAPG2, NCAPH, NDC80, NUSAP1, PRC1, RACGAP1, RRM2, SAC3D1, SPDL1, TOP2A, TPX2, UBE2T
Most correlated modules
- S-phase Replication · correlation 0.95
- Replication-Coupled Histones · correlation 0.93
- Mitotic Spindle · correlation 0.88
- G1/S Entry · correlation 0.81
- DNA Replication Licensing · correlation 0.81
- Cellular Homeostasis · correlation 0.78
Module annotations were drafted by a large language model from the module's genes, then reviewed and approved by a domain expert. See sources & licences.