RUNX3 Tissue Identity
Gene co-expression module in Mucosal-associated invariant T cell
| Category | T cell maturation |
|---|---|
| Genes | 19 |
| Annotation certainty | 3 of 5 |
| Annotation consistency | 10 of 19 genes have a known function matching the annotation |
Why this annotation
The top hub CHD1 is a chromatin remodeler that maintains open chromatin and is linked to gene activation. EP300 (p300) is a major transcriptional co-activator and histone acetyltransferase. KMT2E is a histone methyltransferase. RUNX3 is a transcription factor critical for CD8 T cell and MAIT cell development/maturation and tissue residency. RORA is a nuclear receptor involved in T cell differentiation and circadian regulation. CYLD is a deubiquitinase that negatively regulates NF-kB. SPEN (SHARP/MINT) is a transcriptional repressor. ZBTB1 is a zinc finger transcription factor involved in lymphocyte development. CD44 is a canonical tissue-resident/effector T cell marker. RNF125 is an E3 ubiquitin ligase targeting RIG-I and TCR signaling components. EML4 is a microtubule-associated protein. SLC38A1 is a glutamine transporter (metabolic). B4GALT1 is involved in glycosylation. The module clusters around transcriptional and chromatin regulatory genes that define mature effector/tissue-resident T cell identity, with RUNX3 and CD44 as canonical MAIT/tissue-resident markers. In the context of neighbors M131 (STAT4 identity) and M112 (tissue residency), this neighborhood as a whole appears to define mature MAIT identity and tissue-residency programming.
Genes
B4GALT1, CD44, CDK17, CHD1, CYLD, EML4, EP300, GPBP1, KMT2E, NUDT4, RNF125, RORA, RUNX3, SLC38A1, SPEN, SUCO, TMF1, ZBTB1, ZFC3H1
Most correlated modules
- Rho GTPase Migration · correlation 0.91
- NF-κB RNA Regulation · correlation 0.80
- NF-κB Activation · correlation 0.78
- Chromatin Epigenetic Regulation · correlation 0.77
- cAMP Immune Regulation · correlation 0.76
- Chromatin & Splicing · correlation 0.75
- STAT3 Cytokine Signaling · correlation 0.73
- STAT4 T cell Identity · correlation 0.73
Module annotations were drafted by a large language model from the module's genes, then reviewed and approved by a domain expert. See sources & licences.