NEMF — Nuclear export mediator factor
NEMF belongs to a gene co-expression module in 4 of 28 SCUBA cell types. Each module groups genes that rise and fall together in that cell type; the genes it shares a module with are its closest co-expression partners there.
NEMF's module in each cell type
| Cell type | Module | Shares the module with | |
|---|---|---|---|
| Gamma-delta T cells | Centrosome Organization DNA/chromatin regulation | AKAP9, ARMCX6, CCNT2, CEP350, CUL5, DDHD1, DDX42, DENND6A +23 more | |
| Innate lymphoid cells | Mitochondrial Translation Mitochondrial & OxPhos | ABCF1, ARGLU1, ATG12, BAG1, C1orf35, CHCHD1, CHMP5, DNAJC7 +37 more | View in SCUBA |
| Macrophages | RNA Processing Housekeeping Housekeeping | BAZ1A, BDP1, CCAR1, CCDC88A, CLIP1, CUL5, DHX36, DNM1L +32 more | View in SCUBA |
| Smooth muscle cells | Actin Cytoskeletal Scaffold Cytoskeletal | ACTN4, ATP2B4, ATRX, DDX5, DLGAP4, EIF4G2, FAT1, GNAI2 +13 more | View in SCUBA |
About the gene
| Synonyms | FLJ10051, NY-CO-1, RQC2, SDCCAG1 |
|---|---|
| Chromosome | 14: 49782083-49852821 |
| Predicted location | Intracellular |
| Essential gene | No |
| Protein class | Disease related genes, Human disease related genes, Plasma proteins, Predicted intracellular proteins |
Function
Key component of the ribosome quality control complex (RQC), a ribosome-associated complex that mediates the extraction of incompletely synthesized nascent chains from stalled ribosomes as well as their ubiquitin-mediated proteasomal degradation. Thereby, frees 60S subunit ribosomes from the stalled translation complex and prevents the accumulation of nascent polypeptide chains that are potentially toxic for the cell. Within the RQC complex, NEMF specifically binds stalled 60S ribosomal subunits by recognizing an exposed, nascent chain-conjugated tRNA moiety and promotes the recruitment of LTN1 to stalled 60S subunits. Following binding to stalled 60S ribosomal subunits, NEMF mediates CAT tailing by recruiting alanine-charged tRNA to the A- site and directing the elongation of stalled nascent chains independently of mRNA or 40S subunits, leading to non-templated C- terminal alanine extensions (CAT tails). Mainly recruits alanine-charged tRNAs, but can also other amino acid-charged tRNAs. CAT tailing is required to promote ubiquitination of stalled nascent chains by different E3 ubiquitin-protein ligases. In the canonical RQC pathway (RQC-L), CAT tailing facilitates LTN1-dependent ubiquitination by exposing lysine residues that would otherwise remain buried in the ribosomal exit tunnel (By similarity). In the alternative RQC pathway (RQC-C) CAT tailing creates an C-degron mainly composed of alanine that is recognized by the CRL2(KLHDC10) and RCHY1/PIRH2 E3 ligases, leading to ubiquitination and degradation of stalled nascent chains. NEMF may also indirectly play a role in nuclear export.
Human Protein Atlas · Open Targets · UniProt
Gene annotation from the Human Protein Atlas and UniProt; see sources & licences.